Journal: Journal of visualized experiments : JoVE

Article Title: Chronic Implantation of Multiple Flexible Polymer Electrode Arrays

doi: 10.3791/59957

Figure Lengend Snippet: Materials

Article Snippet: Files available at: https://github.com/jasonechung/PolymerProbe3dParts Dental Acrylic (Hygenic Repair Resin, Coltene type II quick set) Colten/Whaledent 8886784, 8881627 Dental acrylic for use during implant construction Hydraulic Micromanipulator (x2) Narishige Group MO-10 1-axis micromanipulator Kapton Polyimide Tape Bertech PPTDE-1/2 Double-sided tape Kopf Stereotax Arm Kopf Instruments 103088R, 103088L Standard rodent stereotax Light Curable Dental Acrylic, Vivid Flow Coltene/Whaledent D33-01-00 Light curable dental acrylic for use during implant construction Loctite Gel Control Henkel Corp. 234790 1364076 1735574 1752699 Cyanoacrylate for adhering silicon shuttle to corresponding 3d printed part Metabond Quick Cement Parkell S380 For direct application to skull to create strong connection between skull and implant Polymer Electrode Arrays and Silicon Insertion Shuttles Lawrence-Livermore National Laboratory N/A Fabricated at Lawrence-Livermore National Laboratory, polyimide electrode arrays, silicon insertion shuttle Silicone Gel Kit, Low Viscosity Dow Corning Mar-80 Low-viscosity silicone gel for filling of 3d printed base piece Silicone, Medium-Viscosity Kit World Precision Instruments Kwik-Sil Medium-viscosity silicone gel for protection of polymer electrode arrays Open in a separate window Materials This protocol starts with a flexible polymer microelectrode array mounted to a silicon insertion shuttle.

Techniques: Polymer, Viscosity

Journal: bioRxiv

Article Title: Spermidine dampens inflammation by directly inhibiting Th17 cytokine production through a PRDX1 associated antioxidant pathway

doi: 10.1101/2021.08.31.458409

Figure Lengend Snippet: N=5 in both PBS and Spd group. A-B: Lung tissue Il17a and Ifng mRNA (C) and protein (D) productions were obtained by ELISA and qPCR, relative expression to Hprt . C-D: Blockage of spermidine synthesis by DFMO in ALI mice model (N=5 in PBS group; N=4 in DFMO group). IL-17 family production at mRNA level (C) and protein level (D) in lung tissue were obtained by ELISA and qPCR, relative expression to Hprt . E: BAL cells were counted in PBS and spermidine treated lungs from ALI mouse models. F: BAL cell differentiation including monocyte and neutrophil were identified and quantified under the microscope after Kwik-Diff Staining. G: Right middle lung lobe was incubated in a 65C incubator for 48h, wet/dry weight ratio was recorded and edema levels were then calculated by wet weight minus dry weight H: DFMO administration resulted in higher levels of LDH, indicating more inflammation mediated cytotoxicity. I: DFMO group displayed less Monocytes and more Neutrophils in BAL cells. J: DFMO group displayed more severe lung edema, as calculated before in . K: Further lung tissue histology injury score was assessed. Inflammation can be seen in both groups. Compared to the PBS group, Spermidine group displayed less inflammatory cells, slighter alveolar space congestion, and less alveolar wall damage/alveolar fusion. This experiment has been repeated once. L: Further lung tissue histology injury score was assessed. Compared to the PBS group, DFMO group revealed more inflammatory cells infiltration, stronger alveolar space congestion and higher injury score. This experiment has been repeated once.

Article Snippet: Right after centrifuging from the cytocentrifuge (Cytospin 4, Thermo Scientific), fresh BAL cell cytospin slides were stained using a Kwik-Dif Stains kit (Fisher Scientific, #99-907-00) for inflammatory cell differential counts.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Cell Differentiation, Microscopy, Staining, Incubation